John Blakey and Gavin Brown will depart North Melbourne, leaving their role as assistant coaches of the club.
"After conversations with 'Blakes' and 'Browny', we have agreed to mutually part ways," North Melbourne's general manager of football Todd Viney said in a statement.
"These decisions are never taken lightly, but the time is right for all parties to move forward in new directions.
"Blakes and Browny have been outstanding contributors to our club and they leave as highly respected individuals. We wish them well as they look to take on new opportunities within the industry."